Glucose transport machinery reconstituted in cell models

Supplementary information for : Glucose Transport Machinery Reconstituted in Cell Models

Glucose transport in vesicles reconstituted from Saccharomyces cerevisiae membranes and liposomes.

Glucose transport activity was reconstituted into liposomes by the freeze-thaw-sonication procedure from unextracted Saccharomyces cerevisiae membranes and preformed phospholipid liposomes. Fluorescence-dequenching measurements with octadecylrhodamine B chloride (R18)-labeled membranes showed that the yeast membrane lipids are diluted by the liposome lipids after the freeze-thaw-sonication proc...

HIF prolyl hydroxylase PHD3 regulates translational machinery and glucose metabolism in clear cell renal cell carcinoma

BACKGROUND A key feature of clear cell renal cell carcinoma (ccRCC) is the inactivation of the von Hippel-Lindau tumour suppressor protein (pVHL) that leads to the activation of hypoxia-inducible factor (HIF) pathway also in well-oxygenated conditions. Important regulator of HIF-α, prolyl hydroxylase PHD3, is expressed in high amounts in ccRCC. Although several functions and downstream targets ...

A COG in the retrograde transport machinery

PARP retains Ku at double-strand breaks C outo et al. describe how a poly ADP-ribose polymerase (PARP) helps repair DNA double-strand breaks (DSBs) by promoting the accumulation of a key protein required for nonhomologous end joining (NHEJ). PARPs are activated by DNA damage, catalyzing the poly ADP-ribosylation (PARylation) of proteins at both single-strand breaks and DSBs. In vertebrate cells...

Hybrid models of the cell cycle molecular machinery

Piecewise smooth hybrid systems, involving continuous and discrete variables, are suitable models for describing the multiscale regulatory machinery of the biological cells. In hybrid models, the discrete variables can switch on and off some molecular interactions, simulating cell progression through a series of functioning modes. The advancement through the cell cycle is the archetype of such ...